diff --git a/esphome/components/animation/__init__.py b/esphome/components/animation/__init__.py
index 3f03e5c185..1780bdf72e 100644
--- a/esphome/components/animation/__init__.py
+++ b/esphome/components/animation/__init__.py
@@ -60,6 +60,10 @@ async def to_code(config):
             image.seek(frameIndex)
             frame = image.convert("L", dither=Image.NONE)
             pixels = list(frame.getdata())
+            if len(pixels) != height * width:
+                raise core.EsphomeError(
+                    f"Unexpected number of pixels in frame {frameIndex}: {len(pixels)} != {height*width}"
+                )
             for pix in pixels:
                 data[pos] = pix
                 pos += 1
@@ -69,8 +73,14 @@ async def to_code(config):
         pos = 0
         for frameIndex in range(frames):
             image.seek(frameIndex)
+            if CONF_RESIZE in config:
+                image.thumbnail(config[CONF_RESIZE])
             frame = image.convert("RGB")
             pixels = list(frame.getdata())
+            if len(pixels) != height * width:
+                raise core.EsphomeError(
+                    f"Unexpected number of pixels in frame {frameIndex}: {len(pixels)} != {height*width}"
+                )
             for pix in pixels:
                 data[pos] = pix[0]
                 pos += 1