diff --git a/esphome/components/i2s_audio/media_player/__init__.py b/esphome/components/i2s_audio/media_player/__init__.py
index dfc3fb2be2..600a308e6c 100644
--- a/esphome/components/i2s_audio/media_player/__init__.py
+++ b/esphome/components/i2s_audio/media_player/__init__.py
@@ -27,6 +27,7 @@ i2s_dac_mode_t = cg.global_ns.enum("i2s_dac_mode_t")
 CONF_MUTE_PIN = "mute_pin"
 CONF_AUDIO_ID = "audio_id"
 CONF_DAC_TYPE = "dac_type"
+CONF_I2S_COMM_FMT = "i2s_comm_fmt"
 
 INTERNAL_DAC_OPTIONS = {
     "left": i2s_dac_mode_t.I2S_DAC_CHANNEL_LEFT_EN,
@@ -38,6 +39,8 @@ EXTERNAL_DAC_OPTIONS = ["mono", "stereo"]
 
 NO_INTERNAL_DAC_VARIANTS = [esp32.const.VARIANT_ESP32S2]
 
+I2C_COMM_FMT_OPTIONS = ["lsb", "msb"]
+
 
 def validate_esp32_variant(config):
     if config[CONF_DAC_TYPE] != "internal":
@@ -69,6 +72,9 @@ CONFIG_SCHEMA = cv.All(
                     cv.Optional(CONF_MODE, default="mono"): cv.one_of(
                         *EXTERNAL_DAC_OPTIONS, lower=True
                     ),
+                    cv.Optional(CONF_I2S_COMM_FMT, default="msb"): cv.one_of(
+                        *I2C_COMM_FMT_OPTIONS, lower=True
+                    ),
                 }
             ).extend(cv.COMPONENT_SCHEMA),
         },
@@ -94,6 +100,7 @@ async def to_code(config):
             pin = await cg.gpio_pin_expression(config[CONF_MUTE_PIN])
             cg.add(var.set_mute_pin(pin))
         cg.add(var.set_external_dac_channels(2 if config[CONF_MODE] == "stereo" else 1))
+        cg.add(var.set_i2s_comm_fmt_lsb(config[CONF_I2S_COMM_FMT] == "lsb"))
 
     cg.add_library("WiFiClientSecure", None)
     cg.add_library("HTTPClient", None)
diff --git a/esphome/components/i2s_audio/media_player/i2s_audio_media_player.cpp b/esphome/components/i2s_audio/media_player/i2s_audio_media_player.cpp
index 6eaa32c23c..9e2e3f136a 100644
--- a/esphome/components/i2s_audio/media_player/i2s_audio_media_player.cpp
+++ b/esphome/components/i2s_audio/media_player/i2s_audio_media_player.cpp
@@ -148,6 +148,7 @@ void I2SAudioMediaPlayer::start_() {
     pin_config.data_out_num = this->dout_pin_;
     i2s_set_pin(this->parent_->get_port(), &pin_config);
 
+    this->audio_->setI2SCommFMT_LSB(this->i2s_comm_fmt_lsb_);
     this->audio_->forceMono(this->external_dac_channels_ == 1);
     if (this->mute_pin_ != nullptr) {
       this->mute_pin_->setup();
diff --git a/esphome/components/i2s_audio/media_player/i2s_audio_media_player.h b/esphome/components/i2s_audio/media_player/i2s_audio_media_player.h
index dab9a85d7c..092e6de8e8 100644
--- a/esphome/components/i2s_audio/media_player/i2s_audio_media_player.h
+++ b/esphome/components/i2s_audio/media_player/i2s_audio_media_player.h
@@ -39,6 +39,8 @@ class I2SAudioMediaPlayer : public Component, public media_player::MediaPlayer,
 #endif
   void set_external_dac_channels(uint8_t channels) { this->external_dac_channels_ = channels; }
 
+  void set_i2s_comm_fmt_lsb(bool lsb) { this->i2s_comm_fmt_lsb_ = lsb; }
+
   media_player::MediaPlayerTraits get_traits() override;
 
   bool is_muted() const override { return this->muted_; }
@@ -71,6 +73,8 @@ class I2SAudioMediaPlayer : public Component, public media_player::MediaPlayer,
 #endif
   uint8_t external_dac_channels_;
 
+  bool i2s_comm_fmt_lsb_;
+
   HighFrequencyLoopRequester high_freq_;
 
   optional<std::string> current_url_{};