diff --git a/esphome/components/gpio/one_wire/gpio_one_wire.cpp b/esphome/components/gpio/one_wire/gpio_one_wire.cpp
index b4e69e975a..36eaf2160a 100644
--- a/esphome/components/gpio/one_wire/gpio_one_wire.cpp
+++ b/esphome/components/gpio/one_wire/gpio_one_wire.cpp
@@ -94,13 +94,15 @@ bool HOT IRAM_ATTR GPIOOneWireBus::read_bit_() {
 
   // measure from start value directly, to get best accurate timing no matter
   // how long pin_mode/delayMicroseconds took
-  delayMicroseconds(12 - (micros() - start));
+  uint32_t now = micros();
+  if (now - start < 12)
+    delayMicroseconds(12 - (now - start));
 
   // sample bus to read bit from peer
   bool r = pin_.digital_read();
 
   // read slot is at least 60µs; get as close to 60µs to spend less time with interrupts locked
-  uint32_t now = micros();
+  now = micros();
   if (now - start < 60)
     delayMicroseconds(60 - (now - start));